Genome Biology, from Mobile DNA to Chromosome Dynamics
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Inserm U944, CNRS UMR7212, Université de Paris, Institut de Recherche Saint Louis (IRSL)
1 avenue Claude Vellefaux
75475 Paris Cedex 10 FRANCE
Scientific Interest
Transposable Elements (TEs) are major components of eukaryotic genomes. TEs have short-term deleterious effects due to their mobility and presence in multi-copies, leading to genomic instability, particularly in aging cells and malignant contexts. They also play a role in genome evolution by modifying host functions, phenotypes, and gene regulation, and can contribute to the long-term adaptation of organisms to different environments. A critical determinant of the fate of a TE and its impact on the genome is where it initially inserts in the genome. On the other hand, TE transcription is derepressed in yeast stressed cells and in human malignant cells, in which chromosomal rearrangements occur. The lab interests are to decipher the molecular mechanisms that govern TE integration site preferences and to explore how the derepression of TE expression under stress conditions compromises genome stability. We are addressing these questions in the yeast S. cerevisiae with the Ty1 retrotransposon, which targets its integration upstream of Pol III-transcribed genes. We are combining classical molecular genetic approaches with genome wide approaches and single cell microscopy.
Transposable Elements (TEs) are major components of eukaryotic genomes. TEs have short-term deleterious effects due to their mobility and presence in multi-copies, leading to genomic instability, particularly in aging cells and malignant contexts. They also play a role in genome evolution by modifying host functions, phenotypes, and gene regulation, and can contribute to the long-term adaptation of organisms to different environments. A critical determinant of the fate of a TE and its impact on the genome is where it initially inserts in the genome. On the other hand, TE transcription is derepressed in yeast stressed cells and in human malignant cells, in which chromosomal rearrangements occur. The lab interests are to decipher the molecular mechanisms that govern TE integration site preferences and to explore how the derepression of TE expression under stress conditions compromises genome stability. We are addressing these questions in the yeast S. cerevisiae with the Ty1 retrotransposon, which targets its integration upstream of Pol III-transcribed genes. We are combining classical molecular genetic approaches with genome wide approaches and single cell microscopy.
Thematic